I will begin with discussing a microfabricated device technology for single-cell immune function profiling, in particular, the co-detection of 40+ cytokines/chemokines at the level of single cells, representing the highest multiplexing recorded to date for a single-cell protein secretion assay. I will describe how this microdevice called IsoCode was conceived at the beginning, evolved over generations, further integrated with a fully automated single-cell processing platform called IsoLight. These systems have been commercialized and widely used in 70+ cancer centers and pharmaceutical companies to evaluate cancer immunotherapies. In order to dissect single-cell-level omics in the tissue context, we further developed a microfluidic deterministic barcoding technology to perform high-spatial-resolution multi-omics atlas sequencing, which for the first time realized whole transcriptome scale, cellular level (10um), and multi-omic (mRNAs and proteins) mapping of fixed tissue slides. This technology fundamental differs from current spatial transcriptome methods, is highly versatile, and may open a new era of spatial omics with applications in a wide range of biological and biomedical research fields.
Dr. Rong Fan is Professor of Biomedical Engineering at Yale University. He received a B.S. in Applied Chemistry from University of Science and Technology in China, a Ph.D. in Chemistry from the University of California at Berkeley, and then completed the postdoctoral training at California Institute of Technology, prior to launching his own research laboratory at Yale University in 2010. His current interest is focused on developing microtechnologies for single-cell and spatial omics profiling in order to interrogate functional cellular heterogeneity and inter-cellular signaling network in human health and disease (e.g., cancer and autoimmunity). He co-founded IsoPlexis, Singleron Biotechnologies, and AtlasXomics. He served on the Scientific Advisory Broad of Bio-Techne. He is the recipient of numerous awards including the National Cancer Institute’s Howard Temin Career Transition Award, the NSF CAREER Award, and the Packard Fellowship for Science and Engineering. He is a Fellow of the American Institute for Medical and Biological Engineering (AIMBE) and elected a senior member of the National Academy of Inventors (NAI).